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Specimen Validity Test

$0.99

Specimen validity test strips.

This test is used to determine the integrity of human urine prior to a drug test. It checks specific gravity, creatinine, pH value and identifies adulterants which may alter the outcome of the test result.

Features and Benefits.

  • Detects abnormalities not normally found in human urine.
  • Seven different tests for establishing the integrity of human urine.
    1. Creatinine test, checks for a waste product called creatine present in human urine.
    2. Glutaraldehyde, testing for the presence of exogenous aldehyde.
    3. Nitrite tests, checks for commonly used commercial adulterants.
    4. Oxidant test, checks for the presence of oxidants.
    5. pH test, checks for the presence of acidic and alkaline adulterants.
    6. Testing for bleach.
    7. Specific Gravity test, checks for sample dilution.
  • Packaged in packs of 5, 10 and 25 test strips per box..

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SKU: Specimen validity test

Specimen validity test.

Seven different tests establishing the integrity of human urine.

Every drug test should be supported with a specimen validity test. Without this test you can never be certain that the urine sample you are testing for drugs is real or has been tampered with. By using a specimen validity test you can check many aspects of the urine to ensure its integrity. (see ‘Interpreting the test’)

The use of adulterants can cause false negative results either by interfering with the screening test and or by destroying drugs present in the urine. Dilution may also be employed in an attempt to produce false negative result. Dilution of urine with water is probably the simplest urine adulteration method. Bleach, vinegar, Visine, sodium bicarbonate, sodium nitrite, Drano, soft drinks and hydrogen peroxide are the examples of adulterants used to adulterate the urine sample due to cheating the drug test.

Data Sheet

Instructions for testing.

Specimen Collection.

  1. Collect fresh urine from the donor in a clean specimen container.
  2. Test urine sample as soon as possible after collection.
  3. Handle the urine sample as if it is potentially infectious.
  4. Aliquot a small portion of the urine sample into another container for testing in order to avoid contamination of the whole urine sample.
  5. Do not dip specimen test strip directly into the specimen container.

Procedure.

  1. Completely immerse reagent areas of the strip in fresh, well-mixed urine. Remove the strip immediately to avoid dissolving out the reagent areas.
  2. While removing, touch the side of the strip against the rim of the urine container to remove excess urine.
  3. Blot the lengthwise edge of the strip on an absorbent paper towel to further remove excess urine and avoid running over contamination from adjacent reagent pads.
  4. Compare each reagent area to its corresponding color blocks on the color chart and read at the times specified. Proper read time is critical for optimal results.
  5. Obtain results by direct color chart comparison.
  6. If the test indicates adulteration, ask the donor to provide a new sample.
  7. Read all reagent areas between 1 – 2 minutes to determine positive urine from negative urine.
  8. Changes in color after 2 minutes are of no value.

Color comparison provides a semi-quantitative screen for Creatinine, Nitrites, Glutaraldehyde, pH, Specific Gravity, Bleach and Pyridium Chlorochromate in human urine which can help to assess the integrity of the urine sample.

Specimen validity test chart

Quality Control.

For best results, performance of reagent strips should be confirmed by testing known negative and positive specimens or controls whenever a new test is performed or whenever a new bottle is rust opened. Each laboratory should establish its own goals for adequate standard of performance, and should question handling and testing procedures if these standards are not met.

Limitations.

Comparison to the color chart is dependent on the interpretation of the individual. It is therefore, recommended that all laboratory personnel interpreting the results of these strips be tested for color blindness.

Some compounds or physical properties that may affect the test result are listed below. Medications that discolor the urine may also cause abnormal results due to masking of-the-reactions of the reagents on the test pads.

Understanding the results.

In general, all seven tests are based on the chemical reactions of the indicator reagents on the pads with components in the urine sample’ effecting color changes. Results are obtained by comparing the color on each of the test pads with the corresponding pad on the container color chart label.

  • Creatinine: Testing, for sample dilution. In this assay, creatinine reacts with a creatinine indicator in an alkaline condition to form a purplish-brown color complex. The concentration of creatinine is directly proportional to the color intensity of the test pad.
  • Glutaraldehyde: Testing for the presence of exogenous aldehyde. In this assay, the aldehyde group on the glutaraldehyde reacts with an indicator to form a pink/purple color complex.
  • Nitrite: Testing for the presence of exogenous nitrite. Nitrite reacts with an aromatic amine to form a diazonium compound in an acid medium. The diazonium compound in turn couples with an indicator to produce a pink-red/purple color.
  • Oxidants: Testing for presence of oxidizing reagents. In this reaction, a color indicator reacts with oxidants such as hydrogen peroxide, ferricyanide, persulfate, or pyridinium chlorochromate to form a blue color complex. Other colors may indicate the presence of other oxidants.
  • pH: Testing for the presence of acidic or alkaline adulterant. This test is based on the well-known double pH indicator method that gives distinguishable colors over wide pH range.
    The colors- range from orange (low pH) to yellow and green to blue (high pH).
  • Specific Gravity: Testing for sample dilution. This test is based on the apparent pKa change of certain pretreated poly electrolytes in relation to the ionic concentration. In the presence of an indicator, the colors range from dark blue or blue-green in urine of low ionic concentration to green and yellow in urine of higher ionic concentration.
  • Bleach: Testing for the presence of bleach in urine. In this test, the presence of bleach forms a blue-green color complex.
  • Pyridium Chlorochromate: Testing for the presence of chromate in urine. In this test, the presence of chromate forms a blue-green color complex.


Specimen validity test chart

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When it arrives to you depends on which shipping options you’ve chosen and how many items are in the order. Some products are shipped directly from the manufacturer. Shipping for these items is by UPS Ground and usually takes 5 working days to arrive.

Items that we stock are shipped via USPS Priority Mail. Delivery takes 2-5 days from order date.

Shipping restrictions.

We do not ship orders to a P.O. Box, they must be shipped to a street address. You’ll be notified after placing your order if we’re unable to ship specific items to the address you’ve selected.

NOTE: We do not currently to ship orders to Alaska and Puerto Rico.